Nickel pull-down assay protocol 1.준비물 Urea buffer Soluble in water (1L) 8M Urea ->(MW:60.08, 480.64g) 0.3M NaCl ->(MW:58.44, 17.53g) 0.05M Na2HPO4 ->(MW:141.96, 7.098g) 0.05M Tris ->(MW:121.14, 6.057g) 0.001M PMSF ->(MW:174.2, 0.1743g) 0.01M Imidazole ->(MW:68.08, 0.680g) pH 8 Urea wash buffer Soluble in water (urea buffer에서 200ml 가져와서 imidazole을 0.02M로 맞춤, 1M imidazole 용액이있음.) 8M Urea0.3M NaCl0..